Flow Cytometry and Imaging Core

The Flow Cytometry and Imaging Core provides state-of-the-art equipment and expertise in flow cytometry, cell sorting, and imaging. Core Manager Michael Clemente, MS, SCYM(ASCP), a flow cytometrist with extensive experience in immunology and hematology/oncology research, is available for project/experimental design consultation, data analysis, instrument setup, full-service cell sorting, and training. Comprehensive one-on-one training is available to enable independent operation of flow and imaging instruments by investigators and staff.

scope


Flow Cytometry and Cell Sorting Instruments

Analyzers

Center for Immunobiology Flow Room


Attune Nxt Analyzer

16-parameter (14 color) instrument equipped with 4-lasers (488, 640, 405, 561) and a 96 well plate loader for high throughput experiments.

Fortessa Special Order Research Product (SORP) Analyzer, Becton Dickinson

20-parameter (18 color) instrument equipped with 5-lasers (488, 405, 640, 355, 561)

Sorters

equipment


Influx#1 Cell Sorter, Becton Dickinson

16 parameter (14 color) instrument equipped with 4-lasers (488, 640, 405, 355) and 4-way sorting, housed in a Baker BioProtect IV Class II Type A2 biosafety cabinet

Influx#2 Cell Sorter, Becton Dickinson

16 parameter (14 color) instrument equipped with 5-lasers (488, 640, 405, 355, 561) and 6-way sorting, housed in a Baker BioProtect IV Class II Type A2 biosafety cabinet

Melody Cell Sorter

User friendly 11 parameter (9 color) instrument equipped with 3-lasers (488, 405, 640) and 4-way sorting

Microscopy/Imaging Instrumentation

A1R+ Confocal Microscope System, Nikon

Laser-scanning confocal microscope equipped with 405, 488, 561, and 640nm lasers. Galvano scanner enables high-resolution imaging, resonant scanner allows for fast imaging, and the high speed Z-stage allows for efficient Z-stack images. Live cell imaging is available with a CO2 and temperature control system. NIS-Elements C control software enables integrated control of the confocal imaging system, microscope, and peripheral devices with a simple and intuitive interface.

Lionheart FX Automated Live Cell Imager, Biotek

Easy to use, versatile LED based automated fluorescent and phase contrast microscope with accessible filter cubes adaptable for detection of a variety of fluorochromes.

FLoid, ThermoFisher

Located in our tissue culture core, the FLoid is single objective basic 3 color fluorescent microscope with digital zoom, allowing investigators quick assessment of fluorescent intensity